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Male Gipr fl/fl and Gipr Syn / littermates were fed HFD for 13 weeks and then treated with vehicle (2 mL/kg) or mGIPR-Ab (25 mg/kg) every 6 days for 48 days

Tissues were incubated in a 15 ml Falcon tube with 1 ml of collagenase P solution for 15 min at 37 C, followed by addition of 12 ml of the cold G-solution (Sigma Aldrich) and centrifugation at 1,620 rpm at room temperature

Lipopolysaccharide causes an increase in intestinal tight junction permeability in vitro and in vivo by inducing enterocyte membrane expression and localization of TLR-4 and CD14

Lysis of erythrocytes by long-chain acyl esters of carnitine

The roles of glucagon-like peptide-2 and the intestinal epithelial insulin-like growth factor-1 receptor in regulating microvillus length